Introduction RT‑PCR (reverse transcription polymerase chain reaction) converts SARS‑CoV‑2 RNA into complementary DNA and amplifies specific viral gene targets, such as N, E, RdRp or S genes, allowing detection of a few copies of viral material. Because the assay amplifies genetic sequences, it achieves >95 % sensitivity and near‑100 % specificity when specimens—preferably nasopharyngeal swabs—are collected correctly, […]











